The critical role of partially exposed N-terminal valine residue in stabilizing GH10 xylanase from Bacillus sp.NG-27 under poly-extreme conditions.
BACKGROUND: Understanding the mechanisms that govern protein stability under poly-extreme conditions continues to be a major challenge.Xylanase (BSX) from Bacillus sp.NG-27, which has a TIM-barrel structure, shows optimum activity at high temperature and alkaline pH, and is resistant to denaturation by SDS and degradation by proteinase K.A comparat